Quantification of Protein Phosphorylation by Liquid Chromatography−Mass Spectrometry
نویسندگان
چکیده
منابع مشابه
Quantification of Protein Phosphorylation by Liquid Chromatography−Mass Spectrometry
The identification and quantification of specific phosphorylation sites within a protein by mass spectrometry has proved challenging when measured from peptides after protein digestion because each peptide has a unique ionization efficiency that alters with modification, such as phosphorylation, and because phosphorylation can alter cleavage by trypsin, shifting peptide distribution. In additio...
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ژورنال
عنوان ژورنال: Analytical Chemistry
سال: 2008
ISSN: 0003-2700,1520-6882
DOI: 10.1021/ac800337v